Hormone Guide · Spoke 13

Micronutrients and Testosterone: Zinc, Vitamin D, Magnesium, Boron

Zinc, vitamin D, magnesium and boron are seen as the testosterone micronutrients. But what is really behind them? The honest answer is simpler than the marketing promises. With a true deficiency, repleting can play a role. With a full store, more usually does nothing at all.

Shukri Jarmoukli · Physician, Integrative Medicine · ViveCura Berlin
My starting point

Few topics in the men's consultation are as surrounded by advertising as zinc and the rest. Every second drugstore has a powder promising more testosterone. I see it more soberly. Micronutrients are tools, not accelerators. If a building block is missing, repleting it can restart hormone production. If the store is full, more of it usually does nothing. This article works through each of the four nutrients honestly and separates what the studies show from what the tin promises.

Maybe you know the feeling. You feel flatter than before, have read somewhere that zinc or vitamin D are supposed to raise testosterone, and now stand in front of a shelf full of promises. The question that is rarely answered is the most important one: does this do anything for you at all? Because whether a micronutrient can move something almost always depends on whether you were short of it.

In this spoke we look at the four best-known candidates. We understand why zinc, vitamin D, magnesium and boron have something to do with testosterone at the cellular level. We read honestly what controlled trials show for each nutrient. And from that we draw a simple line: repleting a deficiency can make sense, overfilling at normal status rarely does. At the end stand levers that move more than any powder.

Reframe

The interesting question is not whether a micronutrient raises testosterone, but whether you were short of it. An empty store that is refilled can change something. A full store, into which still more is poured, usually changes nothing. That shifts the focus away from the next powder and toward the one question: what am I really missing?

Why micronutrients have anything to do with testosterone at all

Hormone production is handwork at the cellular level. For cholesterol to end up as testosterone, the body needs a chain of enzyme steps, and many of these enzymes only work with the right minerals and vitamins as helpers. If such a helper is missing for a long time, production can stall. That is the core of why micronutrients can play a role for testosterone. But it is also the reason why more is not automatically better.

Zinc in the Leydig cells

Zinc is a building block of many enzymes and docking sites, including in the Leydig cells of the testes, where testosterone is made. At the cellular level, zinc is needed for the control signals and for steps of hormone production. If zinc is markedly missing, testosterone production can suffer. With a full zinc store, by contrast, more zinc brings no extra push. The body does not install more tool than the blueprint calls for.

Vitamin D as a hormone

Vitamin D is strictly speaking not a vitamin but a precursor of a hormone. Docking sites for vitamin D are found in the testes and in the higher control system. This led to the idea that vitamin D might co-steer testosterone production. At the cellular level the mechanism is plausible. Whether it measurably raises testosterone in humans is a different question, and here the data become thin.

Magnesium and the binding protein

Magnesium is involved in hundreds of enzyme steps, including the energy metabolism of muscles and testes. One discussed trail is that magnesium might, at the cellular level, loosen the binding of testosterone to its transport protein SHBG and thus make somewhat more free testosterone available. That is mechanistically interesting, but only weakly supported in human studies and seen mainly in interplay with exercise.

Boron, SHBG and estrogen

Boron is a trace element that can, at the cellular level, influence how the body handles steroid hormones. In small studies, boron shifted the pattern: somewhat more free testosterone, somewhat less binding protein and less estradiol. The mechanism presumably runs through hormone binding and hormone breakdown. Interesting, but built on very little data, which is why special caution is warranted here.

These four docking points explain why the nutrients are in the conversation at all. But they also explain the basic pattern that runs through all the studies in a moment. A tool that is missing can be needed. A tool that is there in abundance brings no added value. And now we look, nutrient by nutrient, at what the data say.

Zinc: the nutrient that is only missing when it is missing

Zinc is the classic among the testosterone micronutrients, and there really is a true basis here. But it lies in the deficiency, not in the surplus. One of the best-known studies shows both sides in a single experiment.

Study · healthy men, zinc status

Zinc deficiency lowered testosterone, repletion raised it again

Cross-section plus experiment, n=40 Ananda Prasad and colleagues studied the relationship between zinc and testosterone in 1996 in Nutrition. In young men whose zinc intake was artificially restricted over several weeks, testosterone dropped clearly. In marginally zinc-poor older men, it rose again after several months of zinc. The authors concluded that zinc can play an important role in the control of testosterone. The frame is important: this was about a true deficiency, not about dosing up in normally supplied men.

Prasad AS, Mantzoros CS, Beck FW, et al. Nutrition. 1996;12(5):344-8. doi:10.1016/s0899-9007(96)80058-x · PMID: 8875519

Exactly this frame decides. When zinc is missing, repleting it can restart hormone production. When the store is full, little happens. This also shows where zinc was added on top of a hormone therapy, without a clear deficiency being present.

Study · men with hypogonadism

Zinc on top of therapy brought hardly more

Randomized, controlled, n=67 Yan-Ling Liu and colleagues studied men with congenital hypogonadotropic hypogonadism in 2017 in Asian Journal of Andrology. One group received the usual hormone therapy, the other additionally zinc. After eighteen months, sperm production was similar in both groups, and the added benefit of zinc was very small. This suggests that, where no marked deficiency is the cause, zinc adds little to good treatment.

Liu YL, Zhang MN, Tong GY, et al. Asian J Androl. 2017;19(3):280-285. doi:10.4103/1008-682X.189621 · PMID: 27768007

Fertility is its own area. Here there are hints that zinc might act more through sperm quality than through the testosterone level. A small controlled study by Hakimeh Akbari and colleagues in 2023 in the Journal of Obstetrics and Gynaecology of India found in infertile men with diabetes that zinc can favourably influence the motility and shape of sperm as well as the stability of the genetic material (doi:10.1007/s13224-023-01767-7, PMID: 37701089). That is a different topic from the testosterone level but belongs to the full picture.

Common misconception

"More zinc, more testosterone." It is not that simple. Zinc is a building block, not an amplifier. With a deficiency, repleting can help, but in a normally supplied man more zinc does not lift testosterone above the normal range. On the contrary: permanently high zinc doses can interfere with copper absorption and thus create new problems. With zinc, the measure counts, not the more.

Vitamin D: much observation, little proof

With vitamin D, the gap between observation and proof is especially large. Observational studies paint a pretty picture, controlled studies dampen it. Both belong to the truth.

Study · men, observation

Higher vitamin D went with higher testosterone

Cross-section, n=2299 Elisabeth Wehr and colleagues evaluated the data of around 2,300 men in 2010 in Clinical Endocrinology. Men with sufficient vitamin D had, on average, higher testosterone and a higher free androgen index than men with a vitamin D deficiency. Striking was a shared annual course: vitamin D and testosterone both reached their peak in August and their low in March. The authors themselves stressed, however, that correlation is not cause and that controlled studies are needed.

Wehr E, Pilz S, Boehm BO, et al. Clin Endocrinol (Oxf). 2009;73(2):243-8. doi:10.1111/j.1365-2265.2009.03777.x · PMID: 20050857

This pattern was confirmed in a large study of Chinese men. Ningjian Wang and colleagues found in 2015 in Reproductive Biology and Endocrinology that a low vitamin D level was associated with a higher rate of hypogonadism, with the link partly explained by body weight and insulin resistance (doi:10.1186/s12958-015-0068-2, PMID: 26177638). So much for the observation. But what happens when you give vitamin D on purpose?

Study · men with low testosterone

Vitamin D did not measurably raise testosterone

RCT, double-blind, n=100 Elisabeth Lerchbaum and colleagues from the group around Stefan Pilz studied 100 healthy men with low testosterone and a low vitamin D level in 2018 in the European Journal of Nutrition. Over twelve weeks, one half received high-dose vitamin D, the other a placebo. The result was clear: vitamin D had no measurable effect on total testosterone or the other hormone values. The same group found, in a second controlled study of healthy men with normal testosterone, likewise no effect on the hormone value.

Lerchbaum E, Trummer C, Theiler-Schwetz V, et al. Eur J Nutr. 2018;58(8):3135-3146. doi:10.1007/s00394-018-1858-z · PMID: 30460609 · and J Clin Endocrinol Metab. 2017;102(11):4292-4302. doi:10.1210/jc.2017-01428 · PMID: 28938446

There is an older, often-cited exception. Stefan Pilz and colleagues found in 2011 in Hormone and Metabolic Research, in overweight, markedly vitamin-D-poor men in a weight loss programme, an increase in testosterone under vitamin D (doi:10.1055/s-0030-1269854, PMID: 21154195). This study was small and intertwined with the weight loss, and the later, cleaner studies from the same research line could not confirm the effect. My honest conclusion: vitamin D is important for bones and the immune system, but as a reliable testosterone booster it is not proven.

Magnesium and boron: interesting trails, thin data

With the next two nutrients it gets interesting and cautious at the same time. There are interesting signals, but the studies are small and rarely replicated. Here the honest separation between mechanism and proof is especially important.

Study · athletes and sedentary men

Magnesium could slightly raise testosterone, especially with exercise

Controlled study, three groups Vedat Cinar and colleagues studied how four weeks of magnesium affect testosterone in 2010 in Biological Trace Element Research. In both sedentary men and martial arts athletes, free and total testosterone rose under magnesium. The increase was stronger in the training men than in the sedentary ones. This suggests that magnesium and exercise play together, but leaves open how much of it is due to repleting a deficiency and how much to the training.

Cinar V, Polat Y, Baltaci AK, Mogulkoc R. Biol Trace Elem Res. 2010;140(1):18-23. doi:10.1007/s12011-010-8676-3 · PMID: 20352370

That is an interesting signal, but no strong proof. The groups were small, and the close link with the training makes it hard to isolate the pure magnesium effect. It is plausible that magnesium helps where it was short before, and that the actual work is done by the strength training. So magnesium can be a building block, but it is not a standalone lever on normal testosterone.

Study · healthy men, boron

Boron shifted the hormone pattern in small groups

Human study, n=8 Mohammad Reza Naghii and colleagues studied the effect of boron in eight healthy men in 2011 in the Journal of Trace Elements in Medicine and Biology. After a week of daily boron, free testosterone rose, while the binding protein SHBG and estradiol fell. Inflammatory markers also went down. That is an interesting signal for how the body handles steroid hormones, but it rests on a very small group without a large confirming study.

Naghii MR, Mofid M, Asgari AR, et al. J Trace Elem Med Biol. 2011;25(1):54-8. doi:10.1016/j.jtemb.2010.10.001 · PMID: 21129941

Against this stands a controlled study that found no boron effect. Nancy Green and Arny Ferrando studied bodybuilders in 1994 in Environmental Health Perspectives, who received boron or a placebo for seven weeks. Both groups increased testosterone, muscle mass and strength, but that was due to the training, not the boron (doi:10.1289/ehp.94102s773, PMID: 7889885). A review by Lara Pizzorno in 2015 in Integrative Medicine places boron as a trace element with diverse effects, but mostly observed at low intakes (PMID: 26770156). My conclusion: boron has interesting mechanisms and weak evidence. The data are not enough for a clear recommendation.

Reframe

With magnesium and boron, it shows well how important the separation between mechanism and proof is. Both have plausible pathways at the cellular level, and individual small studies show signals. But a signal in a small group is not yet a reliable effect. Humility for the uncertain here means: interesting yes, proven no. Whoever can hold that does not fall for every new powder.

What the data show as a red thread

When you lay the four nutrients side by side, a clear pattern emerges. It is not the pattern the advertising tells, but it is honest and it is helpful.

Across all nutrients it holds: repleting a true deficiency can restart hormone production, overfilling a full store usually does nothing. Zinc shows this most clearly, vitamin D likewise; for magnesium and boron the situation is too thin for a verdict. This fits a simple picture: micronutrients are tools of hormone production, not fuel. A tool that is missing can be needed. More tool than needed just lies around unused.

From this follows a reasonable order. First the look at supply and the basics, then targeted repletion if something is missing. Not the other way round. And now you know why the honest answer to the question about the best testosterone supplement is often: it depends on what you are missing.

Common misconception

"If a little helps, then a lot helps even more." With micronutrients that is not true. The dose-response curve is not a straight line. It rises steeply out of the deficiency and flattens as soon as the store is full. Beyond that, more can even harm, for example when high zinc interferes with copper absorption. That is the biological reason why repleting is wise and overdosing is risky.

Three levers that move more than any powder

Before you invest in powder, it is worth looking at the levers that really act on hormone control. They are unspectacular, but they work through exactly the pathways that determine testosterone. These three levers are a start, not a treatment plan. You find the individual path with medical guidance.

1

Eat colourful and whole, instead of guessing gaps

A varied, vegetable- and protein-rich diet usually covers zinc, magnesium and boron on its own. Meat, shellfish, legumes, nuts, seeds and green leafy vegetables are dense sources. Whoever eats this way rarely needs a powder for these nutrients. The plate is, on the topic of micronutrients, often the better pharmacy than the tin, because it delivers the nutrients in their natural network.

2

Train with resistance, because that acts on the hormone

Strength training and regular exercise improve insulin sensitivity, build muscle and reduce belly fat, that is exactly the factors linked with testosterone. In the magnesium data, the effect showed mainly in the training men. This suggests that exercise is the actual lever and a repleted micronutrient only supports it.

3

Measure before you swallow

Because micronutrients can mainly do something with a true deficiency, it is worth knowing your status before you supplement. Vitamin D can be measured well. With zinc, the whole picture from diet, symptoms and findings counts more. Blanket intake on suspicion is rarely wise, because too much of some nutrients can harm. Targeted beats blanket.

And if the symptoms stay despite good basics, then a workup belongs to it that looks at the whole picture, not just one micronutrient. A markedly low testosterone with symptoms belongs in medical hands, including the control hormones, blood count, thyroid and metabolism. That way it can be decided whether the basics are enough or whether more is needed. A good workup takes your symptoms seriously.

The core

Micronutrients fill gaps, they do not build mountains

Zinc, vitamin D, magnesium and boron are tools of your hormone production, not fuel for more. With a true deficiency, repleting can move something. With a full store, more usually does nothing. Save yourself the expensive promise from the tin and invest first where the lever is longest: in sleep, exercise, weight and a colourful diet. Your body does not need mountains, it needs what it is missing.

Frequently asked questions about micronutrients and testosterone

Does zinc raise testosterone?

It depends on your starting status. With a true zinc deficiency, zinc can play a role for testosterone. In a classic study, the testosterone of young men dropped clearly when their zinc intake was artificially restricted over several weeks, and it rose again in marginally zinc-poor older men after repletion. This suggests that zinc is needed for testosterone production. In men with a normal zinc status, by contrast, no reliable effect on testosterone is proven. More zinc than needed does not push testosterone above the normal range. So zinc is not a booster, but a building block that is only missing when it is missing.

Does vitamin D help with testosterone?

The evidence is split and, on the whole, sobering. Observational studies show that men with higher vitamin D levels have, on average, somewhat higher testosterone, with a seasonal parallel across the year. But correlation is not cause. In several controlled trials, vitamin D did not measurably raise testosterone in men with normal or low testosterone. An older study in overweight, markedly vitamin-D-poor men did find an increase, but later, cleaner studies could not confirm it. Vitamin D remains important for bones and the immune system. As a testosterone booster it is not proven.

Can magnesium influence testosterone levels?

Magnesium is involved in hundreds of enzyme steps, including energy metabolism and muscle work. A smaller controlled study found that magnesium over four weeks can slightly raise free and total testosterone, especially in combination with exercise. That is an interesting signal, but the data base is thin, the groups were small, and much suggests the effect runs mainly through repleting a deficiency and through training. Magnesium can make sense when a deficiency is present. As a reliable way to raise normal testosterone further, it is not.

What about boron and testosterone?

Boron is a trace element that can shift the hormone pattern in small human studies. In one study of healthy men, free testosterone rose after a week of boron, while the binding protein SHBG and estradiol fell. That sounds promising, but it rests on very small groups without large, replicated studies. A controlled study in bodybuilders, by contrast, found no boron effect on testosterone. So boron is a nutrient with interesting mechanisms and weak evidence. The data are not enough for a clear recommendation.

Why do micronutrients often do nothing at normal status?

Micronutrients are tools, not accelerators. The body needs zinc, vitamin D or magnesium to make hormones, but it does not make more testosterone just because there is more tool. If a building block is missing, repleting it can restart the disrupted production. If the store is full, more of it brings no extra effect. That explains the pattern running through the studies: repleting a deficiency can help, overfilling at normal status usually does nothing. Whoever understands this saves money on expensive powders and looks at the basics first.

Should I have my levels measured before taking anything?

That is the most sensible step. Because micronutrients mainly do something with a true deficiency, it is worth knowing your status before you supplement. Vitamin D can be measured well in the blood. With zinc, measurement is harder, because the blood value only roughly reflects the tissue store; here the whole picture from diet, symptoms and findings often counts. Blanket intake on suspicion is rarely wise, because too much of some nutrients can harm. Before taking supplements, especially with symptoms, you should have it medically clarified what is really missing.

Can too much zinc harm?

Yes. With zinc, more is not better. High zinc doses over a longer time can interfere with the absorption of copper and thus promote a copper deficiency, which in turn can strain the blood count and the nerves. The stomach also often reacts sensitively. That is a good reason not to take zinc in high doses on your own for long, but rather targeted and time-limited, ideally after medical assessment. The benefit lies in repleting a deficiency, not in permanently overfilling.

What does more than any supplement?

The unspectacular basics. Across all micronutrients, sleep, weight, exercise and stress regulation usually move more for testosterone than a single powder. Strength training, reducing belly fat and restful sleep act directly on the control system that determines testosterone. A varied, vegetable-rich diet also covers most micronutrients on its own. Supplements can close a gap, but they do not replace the basics. Whoever wants to move something for testosterone starts where the lever is longest.

Which foods provide these micronutrients?

Zinc is found mainly in meat, shellfish, cheese, legumes, nuts and seeds. Magnesium is in whole grains, green leafy vegetables, nuts, seeds and dark chocolate. Boron occurs mainly in fruit, nuts and legumes. Vitamin D is an exception, because it is made in the skin through sunlight and is found in only a few foods such as fatty fish. In the dark months a deficiency is therefore common. A colourful, whole-food diet covers most of these nutrients well, which is why the plate is often the better pharmacy than the tin.

Do micronutrients replace treatment for a true testosterone deficiency?

No. With a medically confirmed testosterone deficiency and symptoms, micronutrients are not a treatment. They can offset an accompanying deficiency and support the basics, but they do not lift a markedly lowered testosterone into the normal range. A true deficiency belongs in medical hands, including the control hormones, blood count, thyroid and metabolism. Only then can it be decided whether the basics are enough or whether more is needed. Whoever has persistent symptoms behind it should take them seriously and not tackle them with powders alone.

Connections to other topics

When the value is lowUnderstanding testosterone deficiency

The deeper context of when a low value is really a deficiency and why micronutrients cannot fix it on their own.

When energy is missingIron deficiency and iron infusions

Iron is another micronutrient whose deficiency can trigger exhaustion and reduced stamina that look like a pure hormone problem.

When the thyroid plays alongFunctional hypothyroidism

The thyroid too needs trace elements like iodine and selenium and can co-influence drive, mood and energy.

When stress is the topicCortisol and the HPA axis in burnout

The stress axis draws on micronutrients and is closely intertwined with the control of your testosterone.

When the gut is involvedGut reset: holistic gut treatment

The gut co-decides how well you absorb zinc, magnesium and other micronutrients in the first place.

The other side of hormonesHormonal imbalance in women

How female hormones work as a connected system, with many parallels to the role of micronutrients.

SJ
Written by

Shukri Jarmoukli

Physician, Integrative Medicine, Clinical Psychoneuroimmunology · ViveCura Berlin, Skalitzer Straße 137 · Focus: male hormones as a connected system. With micronutrients I look first at the supply status and the basics before turning to powders. This spoke draws on the research into the link between zinc and testosterone (Prasad 1996, Nutrition), on the controlled vitamin D studies from Graz (Lerchbaum 2017, Journal of Clinical Endocrinology and Metabolism; Lerchbaum 2018, European Journal of Nutrition) as well as on the data on magnesium (Cinar 2010, Biological Trace Element Research) and boron (Naghii 2011, Journal of Trace Elements in Medicine and Biology). My aim is an honest review that separates repleting a deficiency from overfilling at normal status.

Sources and further reading

  1. Prasad AS, Mantzoros CS, Beck FW, Hess JW, Brewer GJ. Zinc status and serum testosterone levels of healthy adults. Nutrition. 1996;12(5):344-8. doi:10.1016/s0899-9007(96)80058-x · PMID: 8875519 [Cohort, Experiment]
  2. Liu YL, Zhang MN, Tong GY, et al. The effectiveness of zinc supplementation in men with isolated hypogonadotropic hypogonadism. Asian J Androl. 2017;19(3):280-285. doi:10.4103/1008-682X.189621 · PMID: 27768007 [RCT]
  3. Akbari H, Elyasi L, Khaleghi AA, Mohammadi M. The effect of zinc supplementation on improving sperm parameters in infertile diabetic men. J Obstet Gynaecol India. 2023;73(4):316-321. doi:10.1007/s13224-023-01767-7 · PMID: 37701089 [RCT]
  4. Wehr E, Pilz S, Boehm BO, März W, Obermayer-Pietsch B. Association of vitamin D status with serum androgen levels in men. Clin Endocrinol (Oxf). 2009;73(2):243-8. doi:10.1111/j.1365-2265.2009.03777.x · PMID: 20050857 [Cohort, Cross-sectional]
  5. Wang N, Han B, Li Q, et al. Vitamin D is associated with testosterone and hypogonadism in Chinese men. Reprod Biol Endocrinol. 2015;13:74. doi:10.1186/s12958-015-0068-2 · PMID: 26177638 [Cohort, Cross-sectional]
  6. Lerchbaum E, Pilz S, Trummer C, et al. Vitamin D and Testosterone in Healthy Men: A Randomized Controlled Trial. J Clin Endocrinol Metab. 2017;102(11):4292-4302. doi:10.1210/jc.2017-01428 · PMID: 28938446 [RCT]
  7. Lerchbaum E, Trummer C, Theiler-Schwetz V, et al. Effects of vitamin D supplementation on androgens in men with low testosterone levels: a randomized controlled trial. Eur J Nutr. 2018;58(8):3135-3146. doi:10.1007/s00394-018-1858-z · PMID: 30460609 [RCT]
  8. Pilz S, Frisch S, Koertke H, et al. Effect of vitamin D supplementation on testosterone levels in men. Horm Metab Res. 2011;43(3):223-5. doi:10.1055/s-0030-1269854 · PMID: 21154195 [RCT]
  9. Cinar V, Polat Y, Baltaci AK, Mogulkoc R. Effects of magnesium supplementation on testosterone levels of athletes and sedentary subjects at rest and after exhaustion. Biol Trace Elem Res. 2010;140(1):18-23. doi:10.1007/s12011-010-8676-3 · PMID: 20352370 [Controlled Clinical Trial]
  10. Naghii MR, Mofid M, Asgari AR, Hedayati M, Daneshpour MS. Comparative effects of daily and weekly boron supplementation on plasma steroid hormones and proinflammatory cytokines. J Trace Elem Med Biol. 2011;25(1):54-8. doi:10.1016/j.jtemb.2010.10.001 · PMID: 21129941 [Cohort, Experiment]
  11. Green NR, Ferrando AA. Plasma boron and the effects of boron supplementation in males. Environ Health Perspect. 1994;102 Suppl 7:73-7. doi:10.1289/ehp.94102s773 · PMID: 7889885 [RCT]
  12. Pizzorno L. Nothing Boring About Boron. Integr Med (Encinitas). 2015;14(4):35-48. PMID: 26770156 [Review]
  13. Midttun M, Overgaard K, Zerahn B, et al. Beneficial effects of exercise, testosterone, vitamin D, calcium and protein in older men. J Cachexia Sarcopenia Muscle. 2024;15(4):1451-1462. doi:10.1002/jcsm.13498 · PMID: 38890228 [RCT]
Note on the evidence: This spoke article combines better-supported with weaker-supported areas. Most solid is the link between zinc deficiency and testosterone (Prasad 1996) as well as the controlled vitamin D studies, which found no effect on the hormone value in men with normal and low testosterone (Lerchbaum 2017, 2018). Observational data show a link between vitamin D and androgens (Wehr 2009, Wang 2015), but prove no cause. For magnesium (Cinar 2010) and boron (Naghii 2011, Green 1994) the data are small and partly contradictory, which is why only interesting signals can be spoken of here, not proven effects. This text serves information and does not replace medical examination, diagnosis or treatment. Micronutrients can make sense with a true deficiency, but they do not replace the workup of a testosterone deficiency. High-dose intake on your own can harm, for example when high zinc interferes with copper absorption. Before taking supplements, especially with symptoms, you should have it medically clarified what is really missing. With persistent, new or unusual symptoms, a medical workup should take place.

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